Murine Model for Small Bowel Resection
The mouse, because of its small size, typically has presented a formidable technical challenge for safe resection and reanastomosis of the small bowel. With the advent of transgenic technology, the mouse is the ideal animal for characterization of adaptation following small bowel resection, because the expression of candidate factors considered essential may be genetically abolished or amplified. Further, transgenic models permit a means to understand temporal, spatial and stimulus-responsive regulation of gene expression. Our laboratory has perfected a murine model for small bowel resection with a resultant survival of greater than 80 percent.
Bernal NP, Stehr W, Coyle R, Erwin CR, Warner BW: EGF receptor signaling regulates Bax and Bcl-w expression and apoptotic responses during intestinal adaptation in mice. Gastroenterology 130: 412-423, 2006.
Allen SR, Ignacio R, Falcone RA, Alonso MH, Brown RL, Garcia VF, Inge TH, Ryckman FC, Warner BW, Azizkhan RG, Tiao GM: The effect of a right-sided aortic arch on outcome in children with esophageal atresia and tracheoesophageal fistula. J Pediatr Surg 41: 479-483, 2006.
Stehr W, Bernal NP, Bernabe KQ, Erwin CR, Warner BW: Absent STAT1 expression perturbs adaptation and apoptosis after massive intestinal resection. J Pediatr Surg 41: 713-718, 2006.
Bernal NP, Stehr W, Zhang Y, Profitt S, Erwin CR, Warner BW: Combined pharmacotherapy that increases proliferation and decreases apoptosis optimally enhances intestinal adaptation. J Pediatr Surg 41: 719-724, 2006.
Stehr W, Bernal NP, Erwin CR, Bernabe KQ, Guo J, Warner BW: Roles for p21waf1/cip1 and p27kip1 during the adaptation response to massive intestinal resection. Am J Physiol Gastrointest Liver Physiol 290: G933-G941, 2006.
Erwin CR, Jarboe MD, Sartor MA, Medvedovic M, Stringer K, Warner BW, Bates MD: Developmental characteristics of adapting mouse small intestine crypt cells. Gastroenterology 130: 1324-1332, 2006.
Warner BW, Erwin CR: Critical roles for EGF receptor signaling during resection-induced intestinal adaptation. J Pediatr Gastroenterol Nutr 43: S68-S73, 2006.
Sheng G, Bernabe KQ, Guo J, Warner BW: Epidermal growth factor receptor mediated proliferation of enterocytes requires p21waf1/cip1 expression. Gastroenterology 131: 153-164, 2006.
Warner BB, Ryan AL, Seeger K, Leonard AC, Erwin C, Warner BW: Ontogeny of salivary epidermal growth factor and necrotizing enterocolitis. J Pediatr 358-363, 2007.
Taylor JA, Bernabe KQ, Guo J, Warner BW: Epidermal growth factor receptor–directed enterocyte proliferation does not induce Wnt pathway transcription. J Pediatr Surg 42: 981-986, 2007.
Sheng G, Guo J, Warner BW: Epidermal growth factor receptor MAPK-dependent activation of Baxasignaling modulates apoptosis via p38 in intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol 293: G599-G606, 2007.
Parvadia JK, Keswani SG, Vaikunth S, Maldonado AR, Marwan A, Stehr W, Erwin C, Uzvolgyi E, Warner BW, Yamano S, Taichman N, Crombleholme TM: Role of VEGF in small bowel adaptation after resection: The adaptive response is angiogenesis dependent. Am J Physiol Gastrointest Liver Physiol 293: G591-G598, 2007.
Tantemsapya N, Meinzner-Derr J, Erwin CR, Warner BW: Body composition and metabolic changes associated with massive intestinal resection in mice. J Pediatr Surg 43:14-19, 2008.
Nair R, Warner BB, Warner BW: Role of EGF and other growth factors in the prevention of necrotizing enterocolitis. Semin Perinatol 32: 107-113, 2008.
Martin CA, Bernabe KQ, Taylor JA, Nair R, Paul RJ, Guo J, Erwin CR, Warner BW: Resection-induced intestinal adaptation and the role of enteric smooth muscle. J Pediatr Surg 43:1011-1017, 2008.
Taylor JA, Martin CA, Nair R, Guo J, Erwin CR, Warner BW: Lessons learned: optimization of the murine small bowel resection model. J Pediatr Surg 43(6):1018-1024, 2008.
Guo J, Longshore S, Nair R, Warner BW: pRb, but not p107 or p130 is required for maintenance of enterocyte quiescence and differentiation in small intestine. J Biol Chem 284: 134-140, 2009.
Longshore SW, Wakeman D, McMellen M, Warner BW: Bowel resection induced intestinal adaptation: Progress from bench to bedside. Minerva Pediatrica 61: 239-251, 2009.
Longshore SW, Nair R, Perrone EE, Erwin CR, Guo J, Warner BW: p21waf1/cip1 deficiency does not perturb the intestinal crypt stem cell population after massive small bowel resection. J Pediatr Surg 44: 1065-1071, 2009.
Martin CA, Perrone EE, Longshore SW, Toste P, Bitter K, Nair R, Guo J, Erwin CR, Warner BW: Intestinal resection induces angiogenesis within adapting intestinal villi. J Pediatr Surg 44: 1077-1083, 2009.
Qiu Z, Longshore SW, Warner BW, Rudnick DA: Murine functional liver mass is reduced following partial small bowel resection. J Gastrointest Surg 13: 2176-2182, 2009.
McMellen ME, Wakeman D, Longshore SW, McDuffie L, Warner BW: Growth factors: possible roles for clinical management of the short bowel syndrome. Semin Pediatr Surg 19: 35-43, 2010
Najaf TA, Vachharajani NA, Warner BW, Vachharajani AJ: Interval between clinical presentation of necrotizing enterocolitis and bowel perforation in neonates. Pediatr Surg Int, 26: 607-609, 2010.
Perrone EE, Chen C, Longshore SW, Okezie O, Warner BW, Sun CC, Alaish SM, Strauch ED: Dietary bile acid supplementation improves intestinal integrity and survival in a murine model. J Pediatr Surg, 45: 1256-1265, 2010.
Wakeman D, Longshore SW, McMellen ME, Santos JA, Guo J, Erwin CR, Warner BW: Extent of small bowel resection does not influence the magnitude of intestinal adaptation in the mouse. J Pediatr Surg 45: 1274-1279, 2010.
McMellen ME, Wakeman D, Erwin CR, Guo J, Warner BW: Epidermal growth factor receptor signaling modulates chemokine (CXC) ligand 5 expression and is associated with villus angiogenesis following small bowel resection. Surgery, In Press 2010